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please try againinclude error,path:/comp/breadcrumb/nav?compId=breadcrumb_nav-15524555348331470 cause:org.springframework.web.util.NestedServletException: Request processing failed; nested exception is java.lang.RuntimeException: org.springframework.transaction.CannotCreateTransactionException: Could not open JDBC Connection for transaction; nested exception is java.sql.SQLException: java.sql.SQLException: An attempt by a client to checkout a Connection has timed out. org.springframework.transaction.CannotCreateTransactionException: Could not open JDBC Connection for transaction; nested exception is java.sql.SQLException: java.sql.SQLException: An attempt by a client to checkout a Connection has timed out. at org.springframework.jdbc.datasource.DataSourceTransactionManager.doBegin(DataSourceTransactionManager.java:245) at org.springframework.transaction.support.AbstractPlatformTransactionManager.getTransaction(AbstractPlatformTransactionManager.java:373) at org.springframework.transaction.interceptor.TransactionAspectSupport.createTransactionIfNecessary(TransactionAspectSupport.java:426) at org.springframework.transaction.interceptor.TransactionAspectSupport.invokeWithinTransaction(TransactionAspectSupport.java:275) at org.springframework.transaction.interceptor.TransactionInterceptor.invoke(TransactionInterceptor.java:96) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.aop.interceptor.ExposeInvocationInterceptor.invoke(ExposeInvocationInterceptor.java:92) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:213) at com.sun.proxy.$Proxy98.getAppByAppId(Unknown Source) at com.alibaba.dubbo.common.bytecode.Wrapper3.invokeMethod(Wrapper3.java) at com.alibaba.dubbo.rpc.proxy.javassist.JavassistProxyFactory$1.doInvoke(JavassistProxyFactory.java:45) at com.alibaba.dubbo.rpc.proxy.AbstractProxyInvoker.invoke(AbstractProxyInvoker.java:71) at com.alibaba.dubbo.config.invoker.DelegateProviderMetaDataInvoker.invoke(DelegateProviderMetaDataInvoker.java:48) at com.alibaba.dubbo.rpc.protocol.InvokerWrapper.invoke(InvokerWrapper.java:52) at com.alibaba.csp.sentinel.adapter.dubbo.SentinelDubboProviderFilter.invoke(SentinelDubboProviderFilter.java:66) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.filter.ExceptionFilter.invoke(ExceptionFilter.java:61) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.filter.TimeoutFilter.invoke(TimeoutFilter.java:41) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.monitor.support.MonitorFilter.invoke(MonitorFilter.java:74) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.protocol.dubbo.filter.TraceFilter.invoke(TraceFilter.java:77) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.filter.ContextFilter.invoke(ContextFilter.java:71) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.filter.GenericFilter.invoke(GenericFilter.java:131) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.filter.ClassLoaderFilter.invoke(ClassLoaderFilter.java:37) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.filter.EchoFilter.invoke(EchoFilter.java:37) at com.alibaba.dubbo.rpc.protocol.ProtocolFilterWrapper$1.invoke(ProtocolFilterWrapper.java:68) at com.alibaba.dubbo.rpc.protocol.dubbo.DubboProtocol$1.reply(DubboProtocol.java:98) at com.alibaba.dubbo.remoting.exchange.support.header.HeaderExchangeHandler.handleRequest(HeaderExchangeHandler.java:96) at com.alibaba.dubbo.remoting.exchange.support.header.HeaderExchangeHandler.received(HeaderExchangeHandler.java:168) at com.alibaba.dubbo.remoting.transport.DecodeHandler.received(DecodeHandler.java:50) at com.alibaba.dubbo.remoting.transport.dispatcher.ChannelEventRunnable.run(ChannelEventRunnable.java:79) at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1145) at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:615) at java.lang.Thread.run(Thread.java:745) Caused by: java.sql.SQLException: java.sql.SQLException: An attempt by a client to checkout a Connection has timed out. at cn.ce.ebiz.framework.persistence.TenantDataSource.getConnection(TenantDataSource.java:137) at org.springframework.jdbc.datasource.DataSourceTransactionManager.doBegin(DataSourceTransactionManager.java:204) ... 40 more Caused by: java.sql.SQLException: An attempt by a client to checkout a Connection has timed out. at com.mchange.v2.sql.SqlUtils.toSQLException(SqlUtils.java:106) at com.mchange.v2.sql.SqlUtils.toSQLException(SqlUtils.java:65) at com.mchange.v2.c3p0.impl.C3P0PooledConnectionPool.checkoutPooledConnection(C3P0PooledConnectionPool.java:527) at com.mchange.v2.c3p0.impl.AbstractPoolBackedDataSource.getConnection(AbstractPoolBackedDataSource.java:128) at org.springframework.jdbc.datasource.lookup.AbstractRoutingDataSource.getConnection(AbstractRoutingDataSource.java:164) at cn.ce.ebiz.framework.persistence.TenantDataSource.getConnection(TenantDataSource.java:111) ... 41 more Caused by: com.mchange.v2.resourcepool.TimeoutException: A client timed out while waiting to acquire a resource from com.mchange.v2.resourcepool.BasicResourcePool@2f4bd2e5 -- timeout at awaitAvailable() at com.mchange.v2.resourcepool.BasicResourcePool.awaitAvailable(BasicResourcePool.java:1317) at com.mchange.v2.resourcepool.BasicResourcePool.prelimCheckoutResource(BasicResourcePool.java:557) at com.mchange.v2.resourcepool.BasicResourcePool.checkoutResource(BasicResourcePool.java:477) at com.mchange.v2.c3p0.impl.C3P0PooledConnectionPool.checkoutPooledConnection(C3P0PooledConnectionPool.java:525) ... 44 more

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化学发光免疫检测中的磁珠

2019/08/20 17:30
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在我们的化学发光免疫检测中,除了常用的发光底物如吖啶酯、抗原、抗体。生物缓冲剂等,还有一个非常重要的原料就是结合抗体的磁珠。这个磁珠也是非常关键的。

其实磁珠不仅仅是用于化学发光免疫检测中,在不同的领域都有广泛的应用。通过在磁珠表面包被上特异性抗体、受体等,用于分离纯化样品中的靶体。磁珠已被广泛应用于免疫分析、核酸分离提取、细胞分选、酶的固定等多个领域。

磁珠一般具有超强的顺磁性,在磁场中能够迅速聚集,离开磁场后又能够有助于磁分离地均匀分散。其次,具有合适的且差别较小的粒径,保证了足够强的磁响应性又不会沉降。再次,具有丰富的表面活性基团,以便可以和生化物质偶联,并在外磁场的作用下实现与被待测样品的分离。与传统的分离方法相比,把磁珠用于生化样品复杂组分的的分离,能够实现分离和富集的同时进行,有效地提高了分离速度和富集效率,同时也使分析检测的灵敏度大大提升。

磁珠的应用领域有:

靶向给药,把指定的药品按照目的所需送到特定的生理部位、器官、组织或者细胞中,并在该位置发挥药物的作用。

热疗,将粒径小的磁珠注射到肿瘤组织,在外加磁场作用下产生能量并均匀释放给肿瘤组织。

磁共振成像对比剂氧化铁纳米颗粒是最常用的MRI对比剂,葡聚糖包覆的氧化铁具有生物相容性,在治疗后可通过肝脏被排泄。

基因载体纳米磁珠作为基因载体,用于基因治疗、细胞转染等。

酶的固定化利用外部的磁场不仅可以控制磁珠固定化酶的运动方式和方向,提高固定化酶的生物相容性和免疫活性,也提高了稳定性和催化效率,而且有助于回收利用,大大降低了成本。

此外,磁珠还用于生物活性物质的分离、食品安全检测,核酸提取,细胞分离,蛋白抗体分离,重金属检测,核磁共振造影剂等方面。

德晟科技自05年就开始研发生产血液检测体外诊断方面试剂,对色原底物新型Trinder's试剂、生物缓冲剂及化学发光试剂有着较深的研究。

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化学发光按照发光时间可分为闪光(Flash)和辉光(Glow)两种,按照法学发光体系可以分为直接化学发光 和酶促化学发光
德晟会是你值得合作的卡波姆供应商。现公司已开始实行批量生产,平均日产量达1t,还有丰富的出口经验,有需要的话可来电详询
根据国内有关媒体报道得知,疫情可能还会在今年的冬天以及明年的春天继续存在或者发展,如果发现了局部地区有疫情情况,应该采取紧急措施,对附近地区人员进行核酸检测,同时对密切接触者进行追踪。另外还要密切关注无症状感染者,这些都是应对疫情最行之有效的措施

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